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Forensic science

Entomology: Changes in hydrocarbon profile of outdoor Calliphora vicina puparial cases

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Forensic science

Entomology: Changes in hydrocarbon profile of outdoor Calliphora vicina puparial cases

Rechten: Alle rechten voorbehouden

Samenvatting

Forensic entomology is the branch of forensic science which focuses on insects, and in this study the hydrocarbons on the cuticle (CHC) of the insects. The hydrocarbons (HC) are on the insects cuticle as a function to prevent dehydration of the insect. Also some studies concluded that the hydrocarbons work as a recognition system in ant colonies. During the growth and subsequently the ageing of the larvae, pupae and adult fly, the hydrocarbon-profile on the cuticle changes over time, but also hydrocarbons are found in the puparial cases after hatching. The species used for getting eggs and larvae for analysis were microscopically identified on fly characteristics. Changes of alkanes with chain length of C17-C35 are found in different combinations in the life cycle. The rearing conditions used for the analyses of larvae seem to have an effect on size and behaviour but the CHC-profile seems really stable for aging the larvae. For this reason the analysis of CHC-profile on larvae seem a good tool to age the larvae, which can be of great importance in forensic aspects.
To plot the results from 10 repeats on every time interval, the peak areas of the hydrocarbons from the chromatograms were put into excel. When the areas of all the compounds were gathered, a principal component analysis (PCA) calculation is done on these results to reduce the amount of data. After PCA these results are plotted and the differences or similarities in HC profile at the time intervals can be seen. The vaporation/degradation of hydrocarbons in the puparial cases exposed to outside conditions, seem to have a similar degradation pattern as the puparial cases from inside conditions, but temperature seems to play a role in the speed of changes in the empty puparial. PCA can be used in different projects and to give an example; the food competition experiment from the first 4,5 months of the internship is reanalysed with PCA and bigger changes can be seen in hydrocarbon profile in larval stages.

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Trefwoorden
OrganisatieAvans Hogeschool
AfdelingATGM Academie voor de technologie van Gezondheid en Milieu
PartnersKeele University
TypeBachelor
TaalEngels

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