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ncRNA in neural cell specification

Identifying mouse homologues in genes of interest

Rechten: Alle rechten voorbehouden

ncRNA in neural cell specification

Identifying mouse homologues in genes of interest

Rechten: Alle rechten voorbehouden

Samenvatting

The neocortex, the largest part of the human brain, is a very complicated structure. The
development
and specification of neural cells is tightly regulated in both time and space of the brain
development. The current model for area specification states that secreted molecules set up a
gradient of transcription factors in neural progenitors which translates into area-specific
neuronal differentiation. The models for when different neurons are produced have mainly based on
studies made in fruit flies. During these studies a predetermined sequence of transcription factors
have been shown to determine what type of neuron is produced at different times and in which order.
There are still unanswered questions regarding these models however, and it is clear that more
regulatory
molecules are involved besides the already known factors. In cells information from DNA is
transcribed into RNA which is then processed and translated into specific proteins. Next to these
protein coding RNAs there are also a lot of different non-coding RNAs (ncRNAs) and the expression
of these are especially enriched in the brain. It was originally thought that these ncRNAs have no
function but in the last decades there has been a change in these thoughts. If they do not have a
function why are they present? It has been discovered by different research teams that some
specific ncRNAs play an important role in different cellular functions. The role in neural cell
specification is however largely unexplored.

The overall aim of this research is to identify the roles of different candidate genes, especially
ncRNAs, in neural cell specification. In this project the following questions have been addressed:
do the candidate ncRNA genes that have been identified in human, have mouse homologues? Can a
method be designed to look for mouse homologues in genes of interest?

The research has been conducted by first taking a full mouse brain and making cell cultures of HeLa
and Hek293 cells. A PCR-based method was set up to:
1: Identify the expression of interesting genes, primary ncRNAs in human cell lines
2: Identifying reciprocal genomic regions in mouse with the expression of potential mouse
homologues.

In chapter 1 the introduction of the research is given. In chapter 2 the used materials and methods
are explained. The results can be read in chapter 3. In chapter 4 the conclusion and discussion are
given. At the end of the report the bibliography can be found.

Toon meer
Trefwoorden
OrganisatieAvans Hogeschool
OpleidingBiologie en Medisch Laboratoriumonderzoek-Breda
AfdelingATGM Academie voor de technologie van Gezondheid en Milieu
PartnersUniversiteit van Stockholm
Datum2014-06-07
TypeBachelor
TaalEngels

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