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Identification of two types of protein immunochemically related to urinary urokinase occurring in human plasma

Identification of two types of protein immunochemically related to urinary urokinase occurring in human plasma

Samenvatting

Plasma urokinase, a plasminogen activator immunochemically related to urinary urokinase (UK), was removed from human plasma (3.5 ng/ml) by immuno-depletion with antibodies raised against UK. The remaining plasminogen activator activity of the depleted plasma could not be inhibited by anti-UK antibodies and a sensitive ELISA for UK did not detect any UK levels that were higher than the background of the assay (0.1 ng/ml). However, when the depleted plasma was subjected to SDS-PAGE, substantial amounts of protein were found hereafter around 110 and 46 kD which now gave a positive reaction in the ELISA (35-350 ng/ml plasma). From these observations it is concluded that in human plasma two types of UK-related protein occur: Type I, among which the plasma urokinase, has antigenic determinants which are directly accessible to the anti-UK antibodies, Type II has determinants in a latent form. The function of the 110 kD type-II protein is that of a plasminogen activator; that of the 46 kD protein is not yet clear.

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OrganisatieHanzehogeschool Groningen
LectoraatBiobased Ingredients and Materials
Gepubliceerd inBiochemical and biophysical research communications Elsevier, Vol. 142, Uitgave: 1, Pagina's: 162-168
Datum1987-01-15
TypeArtikel
ISSN0006-291X
DOI10.1016/0006-291x(87)90465-7
TaalEngels

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