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Isolation and characterization of the viscous, high-molecular-mass microbial carbohydrate fraction from faeces of healthy subjects and patients with Crohn's disease and the consequences for a therapeutic approach

Ruseler-van Embden, J G; Van Lieshout, L M; Binnema, D J; Hazenberg, M P

1. An earlier study by our group revealed that the viscosity of faeces from patients with Crohn's disease is significantly lower than that of healthy subjects. This is due to low concentrations of a high-molecular-mass carbohydrate, probably of bacterial origin. The cause of this phenomenon might be …

1998-10ICT & Media; …Artikel

Chromosomal targeting of replicating plasmids in the yeast Hansenula polymorpha

Faber, K N; Swaving, G J; Faber, F (Life Sciences & Renewable Energy); Ab, G; Harder, W; Veenhuis, M; Haima, P

Using an optimized transformation protocol we have studied the possible interactions between transforming plasmid DNA and the Hansenula polymorpha genome. Plasmids consisting only of a pBR322 replicon, an antibiotic resistance marker for Escherichia coli and the Saccharomyces cerevisiae LEU2 gene were …

1992-11TechniekArtikel

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Isolation and characterization of the viscous, high-molecular-mass microbial carbohydrate fraction from faeces of healthy subjects and patients with Crohn's disease and the consequences for a therapeutic approach

Chromosomal targeting of replicating plasmids in the yeast Hansenula polymorpha

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