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DNA metabarcoding using nrITS2 provides highly qualitative and quantitative results for airborne pollen monitoring

Marcel Polling (Onderzoeker); Melati Sin (Onderzoeker); Letty de Weger (Onderzoeker); Arjen Speksnijder (Lector); Mieke Koenders (Onderzoeker); Hugo de Boer (Onderzoeker); Barbara Gravendeel (Onderzoeker)

Airborne pollen monitoring is of global socio-economic importance as it provides information on presence and prevalence of allergenic pollen in ambient air. Traditionally, this task has been performed by microscopic investigation, but novel techniques are being developed to automate this process. Among …

2022-02-01TechniekArtikel

Lynx presence in Roman times in the lower germanic Limes region

Ivo Horn (Onderzoeker); Aviva Shuman (Student); Irene Chan (Student); Cornelus Maliepaard (Onderzoeker); Lisette Kootker (Onderzoeker); Marcel Eurlings (Onderzoeker); Roderick Bakker (Student); Barbara Gravendeel (Lector); Chiara Cavallo (Onderzoeker)

In 2001 and 2002 an excavation conducted in the town of Alphen aan den Rijn in the Netherlands revealed the vestiges of a Roman fort, Castellum Albaniana, situated along the historical Rhine delta and used for centuries during the Roman occupation (41 AD − 275 CE). Among the animal bones retrieved from …

2021-02-01Natuur & LandbouwArtikel

Mushroom DNA barcoding project

Ivo Horn (Onderzoeker); Peter Verleg (Student); Nafiesa Ibrahim (Student); Khadiedjah Soeleman (Student); Floris van Kampen (Student); Mia Ruesen (Student); Naïsha Reulen (Student); Henk Breij (Student); Roderick Bakker (Student); Barbara Gravendeel (Lector)

DNA barcoding is an important molecular methodology for species identification that was developed over the last two decades and it should be covered inthe biology bachelor curriculum. Here, we present an example of DNA barcoding by sequencing a segment of the 28S nuclear ribosomal large subunitrRNA gene …

2020-05-18Natuur & LandbouwArtikel

Palaeoproteomics of bird bones for taxonomic classification

Ivo Horn (Onderzoeker); Yvo Kenens (Student); Magnus Palmblad (Onderzoeker); Suzanne van der Plas-Duivesteijn (Onderzoeker); Bram Langeveld; Hanneke Meijer; Hans Dalebout; Rob Marissen; Anja Fischer; Vincent Florens; Jonas Niemann; Kenneth Rijsdijk; Anne Schulp; Jeroen F.J. Laros (Onderzoeker); Barbara Gravendeel (Lector)

We used proteomic profiling to taxonomically classify extinct, alongside extant bird species using mass spectrometry on ancient bone-derived collagen chains COL1A1 and COL1A2. Proteins of Holocene and Late Pleistocene-aged bones from dodo (Raphus cucullatus) and great auk (Pinguinus impennis), as well …

2019-04-16Natuur & LandbouwArtikel

Development of a multiplex Q-PCR to detect Trichoderma harzianum Rifai strain T22 in plant roots

Ivo Horn (Onderzoeker); Menno van Rijn (Onderzoeker); Tom J.J. Zwetsloot (Student); Said Basmagi (Student); Anita Dirks-Mulder (Onderzoeker); Willem van Leeuwen (Lector); Willem Ravensberg (Onderzoeker); Barbara Gravendeel (Lector)

The fungal species Trichoderma harzianum is widely used as a biological agent in crop protection. To verify the continued presence of this fungus on plant roots manually inoculated with T. harzianum strain T22, a Q-PCR was designed using specific probes for this particular strain. To develop these molecular …

2016Natuur & LandbouwArtikel

Resistance to Antibiotics and Antifungal Medicinal Products

Esther Kok (Onderzoeker); Miek C. Jong (Onderzoeker); Barbara Gravendeel (Onderzoeker); Willem van Leeuwen (Lector); Erik Baars (Onderzoeker)

The increase of antibiotic resistance worldwide, rising numbers of deaths and costs associated with this, and the fact that hardly any new antimicrobial drugs have been developed during the last decade have increased the interest in Complementary and Alternative Medicine (CAM) therapeutic interventions, …

2015Natuur & LandbouwArtikel

Biodiversiteit als bron van innovatie

Barbara Gravendeel (Lector)

Lectorale rede, zoals uitgesproken door dr. Barbara Gravendeel op 12 november 2013 op Hogeschool Leiden. De collectie van Naturalis Biodiversity Center omvat meer dan 37 miljoen objecten. De kosten voor beheer van en onderzoek aan een dergelijke grote museumcollectie worden steeds hoger. Alle kansen …

2013-11-12Natuur & LandbouwLezing

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