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Alternative primer sets for PCR detection of genotypes involved in bacterial aerobic BTEX degradation

Hendrickx, Barbara; Junca, Howard; Vosahlova, Jolana; Lindner, Antje; Rüegg, Irene; Bucheli-Witschel, Margarete; Faber, Folkert (Life Sciences & Renewable Energy); Egli, Thomas; Mau, Margit; Schlömann, Michael; Brennerova, Maria; Brenner, Vladimir; Pieper, Dietmar H; Top, Eva M; Dejonghe, Winnie; Bastiaens, Leen; Springael, Dirk

Eight new primer sets were designed for PCR detection of (i) mono-oxygenase and dioxygenase gene sequences involved in initial attack of bacterial aerobic BTEX degradation and of (ii) catechol 2,3-dioxygenase gene sequences responsible for meta-cleavage of the aromatic ring. The new primer sets allowed …

2006-02-01AlgemeenArtikel

Isolation of Alcaligenes sp strain L6 at low oxygen concentrations and degradation of 3-chlorobenzoate via a pathway not involving (chloro)catechols

Krooneman, Janneke (Bioconversion And Fermentation Technology); Wieringa, E.B A; Moore, E.R B; Gerritse, J; Prins, R.A; Gottschal, J.C

Isolations of 3-chlorobenzoate (3CBA)-degrading aerobic bacteria under reduced O-2, partial pressures yielded organisms which metabolized 3CBA via the gentisate or the protocatechuate pathway rather than via the catechol route. The 3CBA metabolism of one of these isolates, L6, which,vas identified as …

1996-07-01AlgemeenArtikel

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Alternative primer sets for PCR detection of genotypes involved in bacterial aerobic BTEX degradation

Isolation of Alcaligenes sp strain L6 at low oxygen concentrations and degradation of 3-chlorobenzoate via a pathway not involving (chloro)catechols

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