ALIFE The “all-in-one” method to measure the Total Dietary Fibre content was implemented at the Hanze University of Applies Sciences. Wholemeal bread and crackers showed the expected % of TDF (approx. 6 and10 %, respectively). Enzymatic treatment with a novel starch-modifying enzyme clearly resulted …

ALIFE @ ILST.ALIFE voortgangspresentatie van het CCC- WP2c op 07-02-2011

ALIFE:The “as eaten” method to measure the Total Dietary Fibre content was implemented at the Hanze University of Applied Sciences (WP 4). The enzymatic treatment with the GtfB enzyme clearly resulted in an increased fibre content of starch from 1.6% to approx. 20% (fig A). When using this modified starch …

The “as eaten” method to measure the Total Dietary Fibre content and an in vitro fermentation with colon bacteria were successfully coupled to see if fibre fractions have a prebiotic effect. Similar growth pattern for modified starch, FOS and GOS were observed (Fig A). The qPCR results indicate a significant …

ALIFE @ ILST.ALIFE voortgangspresentatie van het CCC- WP2c op 07-02-2011

Overgewicht is een groeiend probleem. Enerzijds is de mens minder gaan bewegen. Anderzijds is het voedingspatroon drastisch veranderd. De moderne mens consumeert meer (verzadigd) vet, meer dierlijke eiwitten, minder voedingsvezels en minder plantaardige eiwitten. Voedingsvezels wekken de nodige interesse …

Analysis of the bacterial flora is important for the characterization of fermentation events. They help the further validation of the “prebiotic index“ as fast and cost-effective screening of prebotic action within individuals or selected populations.