The amount of used antibiotics is a large problem concerning human health. More and more resistance has occurred during the last years. The Dutch government has announced lowering of used antibiotics in the Netherlands has to be reached. The amount of used antibiotics can be described in several ways. …

Using an optimized transformation protocol we have studied the possible interactions between transforming plasmid DNA and the Hansenula polymorpha genome. Plasmids consisting only of a pBR322 replicon, an antibiotic resistance marker for Escherichia coli and the Saccharomyces cerevisiae LEU2 gene were …