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Production of plasminogen activators and inhibitor by serially propagated endothelial cells from adult human blood vessels

van Hinsbergh, V W; Binnema, D; Scheffer, M A; Sprengers, E D; Kooistra, T; Rijken, D C

Endothelial cells were isolated from arteries and veins obtained from elderly people at autopsy and propagated for 37 to 69 population doublings. The cells secreted tissue-type plasminogen activator (t-PA) and PA inhibitor-1, and, after subculturing, urokinase-type PA (u-PA) antigen. The following differences …

1987-07-01AlgemeenArtikel

Quantitation of urokinase antigen in plasma and culture media by use of an ELISA

Binnema, D J; van Iersel, J J; Dooijewaard, G

An ELISA was set up using polyvinylchloride microtiter plates coated with rabbit anti-UK IgG's and affino-purified goat anti-UK IgG's as second antibody. Detection occurred with rabbit anti-goat IgG antibodies conjugated with alkaline phosphatase. The assay is specific for urokinase (UK) with a detection …

1986-09-01AlgemeenArtikel

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Production of plasminogen activators and inhibitor by serially propagated endothelial cells from adult human blood vessels

Quantitation of urokinase antigen in plasma and culture media by use of an ELISA

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