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Zocht je misschien: genetic, generic?

Microbial bioinformatics for food safety and production

Alkema, Wynand; Boekhorst, Jos; Wels, Michiel; van Hijum, Sacha A F T

In the production of fermented foods, microbes play an important role. Optimization of fermentation processes or starter culture production traditionally was a trial-and-error approach inspired by expert knowledge of the fermentation process. Current developments in high-throughput 'omics' technologies …

2015-06-16AlgemeenAndersoortig materiaal

Diversity in robustness of Lactococcus lactis strains during heat stress, oxidative stress, and spray drying stress

Dijkstra, Annereinou R; Setyawati, Meily C; Bayjanov, Jumamurat R; Alkema, Wynand; van Hijum, Sacha A F T; Bron, Peter A; Hugenholtz, Jeroen

In this study we tested 39 Lactococcus lactis strains isolated from diverse habitats for their robustness under heat and oxidative stress, demonstrating high diversity in survival (up to 4 log units). Strains with an L. lactis subsp. lactis phenotype generally displayed more-robust phenotypes than strains …

2014-01-15AlgemeenArtikel

Genotypic adaptations associated with prolonged persistence of Lactobacillus plantarum in the murine digestive tract

van Bokhorst-van de Veen, Hermien; Smelt, Maaike J.; Wels, Michiel; van Hijum, Sacha A.F.T.; de Vos, Paul; Kleerebezem, Michiel; Bron, Peter A.

Probiotic bacteria harbor effector molecules that confer health benefits, but also adaptation factors that enable them to persist in the gastrointestinal tract of the consumer. To study these adaptation factors, an antibiotic-resistant derivative of the probiotic model organism Lactobacillus plantarum …

2013-05-13AlgemeenArtikel

Prednisolone induces the Wnt signalling pathway in 3T3-L1 adipocytes

Fleuren, Wilco W M; Linssen, Margot M L; Toonen, Erik J M; van der Zon, Gerard C M; Guigas, Bruno; de Vlieg, Jacob; Dokter, Wim H A; Ouwens, D Margriet; Alkema, Wynand

Synthetic glucocorticoids are potent anti-inflammatory drugs but show dose-dependent metabolic side effects such as the development of insulin resistance and obesity. The precise mechanisms involved in these glucocorticoid-induced side effects, and especially the participation of adipose tissue in this …

2013-03-19AlgemeenArtikel

Comparative next-generation mapping of the Phytophthora infestans resistance gene Rpi-dlc2 in a European accession of Solanum dulcamara

Golas, T.M.; van de Geest, H.; Gros, J.; Sikkema, A.; D'Agostino, N.; Nap, Jan Peter; Mariani, C.; Allefs, J.J.H.M.; Rieu, I.

Phytophthora infestans, the causal agent of late blight, remains the main threat to potato production worldwide. Screening of 19 accessions of Solanum dulcamara with P. infestans isolate Ipo82001 in detached leaf assays revealed strong resistance in an individual belonging to accession A54750069-1. This …

2012-08-21TechniekArtikel

Molecular pathway profiling of T lymphocyte signal transduction pathways; Th1 and Th2 genomic fingerprints are defined by TCR and CD28-mediated signaling

Smeets, Ruben L; Fleuren, Wilco W M; He, Xuehui; Vink, Paul M; Wijnands, Frank; Gorecka, Monika; Klop, Henri; Bauerschmidt, Sussane; Garritsen, Anja; Koenen, Hans J P M; Joosten, Irma; Boots, Annemieke M H; Alkema, Wynand

BACKGROUND: T lymphocytes are orchestrators of adaptive immunity. Naïve T cells may differentiate into Th1, Th2, Th17 or iTreg phenotypes, depending on environmental co-stimulatory signals. To identify genes and pathways involved in differentiation of Jurkat T cells towards Th1 and Th2 subtypes we performed …

2012-03-14AlgemeenArtikel

Highlights from the 7th European meeting on molecular diagnostics

Anne Loonen (Lid Lectoraat); Rob Schuurman; Adriaan J.C. van den Brule (Lector)

This report presents the highlights of the 7th European Meeting on Molecular Diagnostics held in Scheveningen, The Hague, The Netherlands, 12-14 October 2011. The areas covered included molecular diagnostics applications in medical microbiology, virology, pathology, hemato-oncology,clinical genetics …

2012Natuur & LandbouwArtikel

Prednisolone-induced beta cell dysfunction is associated with impaired endoplasmic reticulum homeostasis in INS-1E cells

Linssen, Margot M L; van Raalte, Daniel H; Toonen, Erik J M; Alkema, Wynand; van der Zon, Gerard C; Dokter, Wim H; Diamant, Michaela; Guigas, Bruno; Ouwens, D Margriet

Glucocorticoids (GCs), such as prednisolone (PRED), are widely prescribed anti-inflammatory drugs, but their use may induce glucose intolerance and diabetes. GC-induced beta cell dysfunction contributes to these diabetogenic effects through mechanisms that remain to be elucidated. In this study, we hypothesized …

2011-11-01AlgemeenArtikel

Literature mining for the discovery of hidden connections between drugs, genes and diseases

Frijters, Raoul; van Vugt, Marianne; Smeets, Ruben; van Schaik, René; de Vlieg, Jacob; Alkema, Wynand

The scientific literature represents a rich source for retrieval of knowledge on associations between biomedical concepts such as genes, diseases and cellular processes. A commonly used method to establish relationships between biomedical concepts from literature is co-occurrence. Apart from its use …

2010-09-23AlgemeenArtikel

Saturation mutagenesis reveals the importance of residues alphaR145 and alphaF146 of penicillin acylase in the synthesis of beta-lactam antibiotics

Jager, Simon A W; Shapovalova, Irina V; Jekel, Peter A; Alkema, Wynand B L; Svedas, Vytas K; Janssen, Dick B

Penicillin acylase (PA) from Escherichia coli can catalyze the coupling of an acyl group to penicillin- and cephalosporin-derived beta-lactam nuclei, a conversion that can be used for the industrial synthesis of beta-lactam antibiotics. The modest synthetic properties of the wild-type enzyme make it …

2008-01-01AlgemeenArtikel

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