PCR-free techniques such as meta-mitogenomics (MMG) can recover taxonomic composition of macroinvertebrate communities, but sufer from low efciency, as >90% of sequencing data is mostly uninformative due to the great abundance of nuclear DNA that cannot be identifed with current reference databases. …

Spatial and temporal patterns in submarine groundwater discharge (SGD) of nitrate in a high-energy sandy beach system on Spiekeroog Island were examined utilizing UV photometry. SGD as pathway of nitrate was studied to gain better understanding of the variability of nitrate discharge and the factors …

Caenorhabditis elegans is an invertebrate model organism used in many areas of biology including developmental biology and the identification of molecular mechanisms and pathways. However, several experimental approaches require large quantities of worms, which is limiting and time-consuming. We present …

The two-year education programme HiPerGreen initiated by Inholland University of Applied Sciences came to a closure on Friday 27th September 2019 at the World Horti Center in Naaldwijk. The Final symposium gave consortium partners and other guests the opportunity to learn about the HiPerGreen’s team …

UPLC-MS is a commonly used technique to first separate complex samples and subsequently quantify molecules of interest. Herein we describe the use of UPLC-MS using an amide stationary phase to quantify non-derivatized amino acids extracted from fingerprints. As detector either a triple-quadrupole MS/MS …

What about people, hardware (biology) and software (culture)? Our construction starts with an egg cell and a sperm cell that is 83,000 times smaller (in this phase the female is certainly cooperative). The 2 cells double, and double, 47 times (247) resulting in the 140.737 billion (1.4 * 1014) complex …

This study aimed to get more insight into the spatial distribution pattern of the macrobenthos at the Vlakte van de Raan in relation to environmental gradients (median grain size, salinity and bathymetry). 82 samples were collected in November 2016 with a Van Veen grab using the research vessel the ‘RV …

This article describes a relatively straightforward procedure to knock out the gene that encodes the invertase enzyme in baker’s yeast. The SUC2 gene, which encodes for the invertase enzyme, is knocked out by a single-step PCR knock out method. The knockout is subsequently confirmed at the genetic level …

Carnitine/choline acyltransferases play diverse roles in energy metabolism and neuronal signalling. Our knowledge of their evolutionary relationships, important for functional understanding, is incomplete. Therefore, we aimed to determine the evolutionary relationships of these eukaryotic transferases. …

Students, researchers, professors and company representatives gathered to share progress and ideas at the second half-year symposium of HiPerGreen at the World Horti Center in Naaldwijk. HiPerGreen is a Raak MKB subsidised research project aiming to bring value to horticultural growers. Cock Heemskerk, …