Using an optimized transformation protocol we have studied the possible interactions between transforming plasmid DNA and the Hansenula polymorpha genome. Plasmids consisting only of a pBR322 replicon, an antibiotic resistance marker for Escherichia coli and the Saccharomyces cerevisiae LEU2 gene were …

Oral flow was registered with a Rothenberg mask in two male subjects during the utterance of an /æ/ vowel embedded in a word at three intensity levels (soft, normal, and loud voice), and during the production of crescendos and decrescendos on the same vowel at three fundamental frequencies. Registered …