DNA barcoding is an important molecular methodology for species identification that was developed over the last two decades and it should be covered inthe biology bachelor curriculum. Here, we present an example of DNA barcoding by sequencing a segment of the 28S nuclear ribosomal large subunitrRNA gene …

Montmorency Tart Cherries, MTC, (Prunus cerasus L.) possess a high anthocyanin content as well as one of the highest oxygen radical absorbance capacities of fruits at common habitual portion sizes. MTC have been shown to contribute to reducing plasma lipids, plasma glucose and fat mass in rats and strikingly, …

DNA-based identification through the use of metabarcoding has been proposed as the next step in the monitoring of biological communities, such as those assessed under the Water Framework Directive (WFD). Advances have been made in the field of metabarcoding, but challenges remain when using complex samples. …

The amino acid profile obtained from a fingerprint may provide valuable information on its donor. Unfortunately, the collection of chemicals from the fingerprint is often destructive to the fingerprint ridge detail. Herein we detail the use of cross-linkable solutions of dextran-methacrylate to form …

PCR-free techniques such as meta-mitogenomics (MMG) can recover taxonomic composition of macroinvertebrate communities, but sufer from low efciency, as >90% of sequencing data is mostly uninformative due to the great abundance of nuclear DNA that cannot be identifed with current reference databases. …

This research used a newly developed, full-scale infiltration testing (FSIT) procedure to determine the saturated surface infiltration rate of 16 existing permeable pavement installations in the Netherlands that have been in service for a number of years. Newly installed permeable pavements in the Netherlands …

Caenorhabditis elegans is an invertebrate model organism used in many areas of biology including developmental biology and the identification of molecular mechanisms and pathways. However, several experimental approaches require large quantities of worms, which is limiting and time-consuming. We present …

Canalization, an intrinsic robustness of development to external (environmental) or internal (genetic) perturbations, was first proposed over half a century ago. However, whether the robustness to environmental stress (environmental canalization [EC]) and to genetic variation (genetic canalization) are …

The world population is aging, which increases the prevalence of physical limitation and chronic diseases. A major cause of these physical limitation and chronic diseases is a low muscle mass (sarcopenia) and/or a high fat mass (obesity). Coexistence of sarcopenia and obesity, called sarcopenic obesity, …

UPLC-MS is a commonly used technique to first separate complex samples and subsequently quantify molecules of interest. Herein we describe the use of UPLC-MS using an amide stationary phase to quantify non-derivatized amino acids extracted from fingerprints. As detector either a triple-quadrupole MS/MS …