DNA barcoding is an important molecular methodology for species identification that was developed over the last two decades and it should be covered inthe biology bachelor curriculum. Here, we present an example of DNA barcoding by sequencing a segment of the 28S nuclear ribosomal large subunitrRNA gene …

MSc. course “Molecular Mechanisms of Disease” - ‘Excellence in Communication’

A Mycobacterial Antacid

The amino acid profile obtained from a fingerprint may provide valuable information on its donor. Unfortunately, the collection of chemicals from the fingerprint is often destructive to the fingerprint ridge detail. Herein we detail the use of cross-linkable solutions of dextran-methacrylate to form …

PCR-free techniques such as meta-mitogenomics (MMG) can recover taxonomic composition of macroinvertebrate communities, but sufer from low efciency, as >90% of sequencing data is mostly uninformative due to the great abundance of nuclear DNA that cannot be identifed with current reference databases. …

Opening Health Hub Roden

Caenorhabditis elegans is an invertebrate model organism used in many areas of biology including developmental biology and the identification of molecular mechanisms and pathways. However, several experimental approaches require large quantities of worms, which is limiting and time-consuming. We present …

UPLC-MS is a commonly used technique to first separate complex samples and subsequently quantify molecules of interest. Herein we describe the use of UPLC-MS using an amide stationary phase to quantify non-derivatized amino acids extracted from fingerprints. As detector either a triple-quadrupole MS/MS …

Symposium “A Chemical Journey” van de studentenvereniging “De Chemische Binding”.