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Comparative study using phenotypic, genotypic and proteomics methods for identification of coagulase-negative staphylococci

Anne J. M. Loonen (Lid Lectoraat); Arjan R. Jansz; Jeandery N. B. Bergland; Marion Valkenburg; Petra F. G. Wolffs; Adriaan J. C. van den Brule (Lector)

Five methods were compared to determine the best technique for accurate identification of coagulase-negative staphylococci (CoNS) (n=142 strains). MALDI-TOF MS showed the best results for rapid and accurate CoNS differentiation (correct identity in 99.3%). An alternative to this approach could be Vitek2 …

2012-03-16Natuur & LandbouwArtikel

An evaluation of three processing methods and the effect of reduced culture times for faster direct identification of pathogens from BacT/ALERT blood cultures by MALDI-TOF MS

A.J.M. Loonen (Lid Lectoraat); A.R. Jansz; J. Stalpers; P.F.G. Wolffs; A.J.C. van den Brule (Lector)

Matrix-assisted laser desorption/ionisation time of-flight mass spectrometry (MALDI-TOF MS) is a fast and reliable method for the identification of bacteria from agar media. Direct identification from positive blood cultures should decrease the time to obtaining the result. In this study, three different …

2011-11-12Natuur & LandbouwArtikel

Acceleration of direct identification of S.aureus versus Coagulase Negative Staphylococci from blood culture material: a comparison of six bacterial DNA extraction methods

A.J.M. Loonen (Lid Lectoraat); A.R. Jansz; H. Kreeftenberg; C. A. Bruggeman; P.F.G. Wolffs; A. J. C. van den Brule (Lector)

To accelerate differentiation between Staphylococcus aureus and Coagulase Negative Staphylococci (CNS), this study aimed to compare six different DNA extraction methods from 2 commonly used blood culture materials, i.e. BACTEC and Bact/ALERT. Furthermore, we analyzed the effect of reduced blood culture …

2010-10-24Natuur & LandbouwArtikel

Vorige Volgende

Comparative study using phenotypic, genotypic and proteomics methods for identification of coagulase-negative staphylococci

An evaluation of three processing methods and the effect of reduced culture times for faster direct identification of pathogens from BacT/ALERT blood cultures by MALDI-TOF MS

Acceleration of direct identification of S.aureus versus Coagulase Negative Staphylococci from blood culture material: a comparison of six bacterial DNA extraction methods

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