HBO Kennisbank

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Zocht je misschien: dnaj, dnab, bacteria, bacterien?

Uncovering bacterial and functional diversity in macroinvertebrate mitochondrial-metagenomic datasets by diferential centrifugation

Jan-Niklas Macher (Onderzoeker); Arjen Speksnijder (Lector); Le Qin Choo (Onderzoeker); Berry van der Hoorn (Onderzoeker); Willem Renema (Onderzoeker)

PCR-free techniques such as meta-mitogenomics (MMG) can recover taxonomic composition of macroinvertebrate communities, but sufer from low efciency, as >90% of sequencing data is mostly uninformative due to the great abundance of nuclear DNA that cannot be identifed with current reference databases. …

2019-07-16Natuur & LandbouwArtikel

Diversity in robustness of Lactococcus lactis strains during heat stress, oxidative stress, and spray drying stress

Dijkstra, Annereinou R; Setyawati, Meily C; Bayjanov, Jumamurat R; Alkema, Wynand (Data Science For Life Science & Health); van Hijum, Sacha A F T; Bron, Peter A; Hugenholtz, Jeroen

In this study we tested 39 Lactococcus lactis strains isolated from diverse habitats for their robustness under heat and oxidative stress, demonstrating high diversity in survival (up to 4 log units). Strains with an L. lactis subsp. lactis phenotype generally displayed more-robust phenotypes than strains …

2014-01-15AlgemeenArtikel

Enzymatic degradation of granular potato starch by Microbacterium aurum strain B8.A

Sarian, Fean D; van der Kaaij, Rachel M; Kralj, Slavko; Wijbenga, Dirk-Jan; Binnema, Doede J.; van der Maarel, Marc J E C; Dijkhuizen, Lubbert

Microbacterium aurum strain B8.A was isolated from the sludge of a potato starch-processing factory on the basis of its ability to use granular starch as carbon- and energy source. Extracellular enzymes hydrolyzing granular starch were detected in the growth medium of M. aurum B8.A, while the type strain …

2012-01AlgemeenArtikel

Acceleration of direct identification of S.aureus versus Coagulase Negative Staphylococci from blood culture material: a comparison of six bacterial DNA extraction methods

A.J.M. Loonen (Lid Lectoraat); A.R. Jansz; H. Kreeftenberg; C. A. Bruggeman; P.F.G. Wolffs; A. J. C. van den Brule (Lector)

To accelerate differentiation between Staphylococcus aureus and Coagulase Negative Staphylococci (CNS), this study aimed to compare six different DNA extraction methods from 2 commonly used blood culture materials, i.e. BACTEC and Bact/ALERT. Furthermore, we analyzed the effect of reduced blood culture …

2010-10-24Natuur & LandbouwArtikel

PCR-DGGE method to assess the diversity of BTEX mono-oxygenase genes at contaminated sites

Hendrickx, Barbara; Dejonghe, Winnie; Faber, Folkert (Life Sciences & Renewable Energy); Boënne, Wesley; Bastiaens, Leen; Verstraete, Willy; Top, Eva M; Springael, Dirk

tmoA and related genes encode the alpha-subunit of the hydroxylase component of the major group (subgroup 1 of subfamily 2) of bacterial multicomponent mono-oxygenase enzyme complexes involved in aerobic benzene, toluene, ethylbenzene and xylene (BTEX) degradation. A PCR-denaturing gradient gel electrophoresis …

2006-02-01AlgemeenArtikel

Alternative primer sets for PCR detection of genotypes involved in bacterial aerobic BTEX degradation

Hendrickx, Barbara; Junca, Howard; Vosahlova, Jolana; Lindner, Antje; Rüegg, Irene; Bucheli-Witschel, Margarete; Faber, Folkert (Life Sciences & Renewable Energy); Egli, Thomas; Mau, Margit; Schlömann, Michael; Brennerova, Maria; Brenner, Vladimir; Pieper, Dietmar H; Top, Eva M; Dejonghe, Winnie; Bastiaens, Leen; Springael, Dirk

Eight new primer sets were designed for PCR detection of (i) mono-oxygenase and dioxygenase gene sequences involved in initial attack of bacterial aerobic BTEX degradation and of (ii) catechol 2,3-dioxygenase gene sequences responsible for meta-cleavage of the aromatic ring. The new primer sets allowed …

2006-02-01AlgemeenArtikel

Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage

Krooneman, Janneke; Faber, F (Biorefinery); Alderkamp, AC; Elferink, SJHWO; Driehuis, F; Cleenwerck, [No Value]; Swings, J; Gottschal, JC; Vancanneyt, M

Inoculation of maize silage with Lactobacillus buchneri (5 × 105 c.f.u. g-1 of maize silage) prior to ensiling results in the formation of aerobically stable silage. After 9 months, lactic acid bacterium counts are approximately 1010 c.f.u. g-1 in these treated silages. An important subpopulation (5.9 …

2002-03-01AlgemeenArtikel

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